plate reader molecular devices. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. plate reader molecular devices

 
SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Revplate reader molecular devices  Plate pre-read measurements were made in a Costar UV plate

Patented pathlength correction for better absorbance accuracy Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. 5 µL of each sample was pipetted into a 96-well. With the discontinuous kinetics feature in SoftMax Pro Software. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. . Three low volume 24-well bottom slides. 005 EU/mL to 5. The ultimate guide to microplate readers is an eBook that tells you everything you need to know. Laboratory automation workflows can help. 2. Turn the reader on and secure the USB cable. Plate Reader-2 features a wide range of high-performance multi-mode reader capabilities ideal for life science research on the International Space Station (ISS. absorbance plate readers. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The former have been the traditional domain of plate readers and great strides have been. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 0 . The FlexStation® Scanning Fluorometer (Molecular Devices; Fig. What are the Vmax plate reader specifications? Recommended Articles SoftMax® Pro 7. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. together with a Molecular Devices plate. Select the Driver tab. Also refer to the Multimode Reader Comparison Chart Features. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Optimized optical path - The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. A). Price Get Quote. Since cells are nearly confluent, the protocol. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Set up the instrument and software. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. This document is provided to customers who have purchased Molecular Devices, Inc. Supplier: Molecular Devices. DOCUMENTATION. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Find our technical support information along with contact details. Ensure data accuracy of your reader. 1). 1. The adapter is required for optimum performa nce with standard 96-well and 384-. Molecular Devices SpectraMax Gemini XS Microplate Fluorometer. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. SoftMax® Pro Software Version 5 for Mac and Windows User Guide 5000938 B June 2010 SoftMaxPro_UserGuide_5000938. The Versamax Microplate Reader is built around a grating monochromator. Molecular Devices Software. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of. The computer controlling the plate reader runs Windows XP. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated—any potential issuesPlate reader instructions Computer. Optimize. Figure 1. Place the microplate in the drawer. Incubate at RT. Read times. Instrument settings can be saved as a protocol file. The FlexStation 3 Reader combines Molecular Devices’ SpectraMax® M5e Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). ~ Bio-Tek ELx808 Microplate Reader $2,795. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes wi. Measure more accurately. Eight filter options provide simplified assay setup. Molecular Devices makes. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. 1. Double-click the unknown device with the yellow warning icon. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. It will operate in 96 and 384 well plate format, and has installed filters for fluorescence of 370, 485 and 535 nm for excitation. Please Inquire. Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Shop a wide selection of Molecular Devices FLIPR TETRA System's on LabX. This document is provided to customers who have purchased Molecular Devices, Inc. VMax® Microplate Readers. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Figure 9. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. The test compound menadione was added to cells in a 1:3. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Versa. EMax. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Plate Reader. and fluorescence microplate readers—. Wait 20 seconds. No accessories are needed and SoftMax® Pro Software. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. SpectraTest ABS2 Absorbance Validation Plate SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e,. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. The Back Panel Figure 1. 2. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). Consider non binding surface type plate (Greiner and Corning are 2 manufacturers)Molecular Devices microplate readers. A. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial. Superior optical system includes a xenon flash lamp and features. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. 2. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. We would like to show you a description here but the site won’t allow us. Endpoint readers are prolific in the laboratory since absorbance has become the detection of choice for many applications. A preconfigured. #4TI-0214) UltraVision Plate 96 (Brooks Life. After reading the ELISA plate on both readers, each standard curve was nearly identical. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Here, the CVs for each plate were less than 3, and the difference between mean onset time for the plates run on the two readers was only 7%. Lab automation for high-throughput plate-based assays. The plate was incubated at room temperature for 50 minutes or 2. Shortside barcode reader: 0310-5102. 340 PC384. Are you looking for a versatile and affordable microplate reader that can perform various assays and measurements? Check out the FilterMax F3 and F5 Multi-Mode Microplate Readers, which offer absorbance, fluorescence, luminescence, and more read modes with high performance and reliability. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. The adapter is required for optimum performa nce with standard 96-well and 384-. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. The plate was then read on Molecular Devices microplate readers with luminescence detection mode (Read A). Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. FLIPR. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. , target CV per well = 1. tools to expand the boundaries of their research. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. #0200-7011) Instrument setupSOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. As new assays are developed or your needs change, simply add a detection cartridge like ScanLater™ Western Blot System or the SpectraMax® MiniMax™ Imaging Cytometer option as opposed to buying a brand new system. NORTH AMERICA. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. One plate carrier adapter is provided with the instrument. Page 1 of 8 . The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. 2. PathCheck sensor corrects optical pathlength & pipetting variation. $2,800. 5: Schematic of the back panel of a reader. PURPOSE. sensitivity or performance. Molecular Devices' microplate readers. 0 . The adapter is required for optimum performance with standard 96-well and 384-. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. The plate is set up for reading and images are acquired according to specified parameters. For more sample throughput, the system can be easily integrated into a full robotic to that of dedicated single-mode readers. . It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Cells were thawed and propagated according to the recommended method specified by the supplier. Wait 20 seconds. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. 50-plate magazine : 0200-6187. Robust, high value microplate reader designed to deliver research-grade results to any laboratory. Description Figure 1. ELISA Plate Readers / Microplate Readers. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. 303 unable to cal dark current. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. SoftMax® Pro 7. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. An example of an upgradeable multi-mode plate reader is the SpectraMax M5e, a five-mode reader that is part of the same line as the first microplate reader used in space, thanks to its durability. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. 9. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. Molecular Devices, LLC Optical System: Inquire; Measuring Time: White light - 96-well/ 384 well microplate: <2 min Fluorescence - 96-well/ 384 well microplate: <8 min. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer. Molecular Devices’ unique SpectraDrop™ Micro-Volume Plate offers a high throughput and accurate solution for low volume measurement. Recertification service. BioTek Synergy H1 Hybrid OMRF research scientist Kumar Subramani reviewed BioTek Synergy H1, stating it is a configurable multi-mode microplate reader. In addition, users can define individual reagents and concentrations to be delivered to each well. Your applications, your modes, your choice. The VMax® Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm–750nm), with 4 filter choices included at time of purchase. Cellular Imaging Systems. The PyroGene assay has been optimized to be linear from 0. US HEADQUARTERS. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 40266 Email: drugdiscoverytech@lifetech. 2. White plates reflect the light, enabling even low levels of Luminescence to be detected. Molecular Devices' microplate readers. 0. Our microplate readers are the industry’s most cited instruments and have empowered life science researchers to advance protein and cell biology, breaking the barriers to novel, landmark discoveries. Uncontrolled Copy for Training and Reference Purposes Only . The plate reader. Description 1. Reader Documentation Version No. SpectraMax Microplate Readers. (7) Anastellin impacts on the processing of extracellular matrix fibronectin and stimulates release of cytokines from coronary artery smooth muscle cells. Molecular Devices SpectraMax 384 Plus Microplate Reader. please click here. Flexibility to read a variety of plate formats. 0 × 103 to 2. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. the iD5 reader’s filter system. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Catalog Number: (89212-396) Supplier: Molecular Devices. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aPrimary considerations when choosing a microplate reader are throughput and flexibility. S. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. One plate carrier adapter is provided with the instrument. Page 1 of 8 . The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. Fortunately, Molecular Devices’ SpectraMax i3x and iD5 readers offer the best of both worlds. Turn on Plate Reader and wait for it to complete its start-up routine. OPTICS Mirrored optics focus the light into the sample volume, and cutoff filters are used to. gene expression with a user-friendly reader. integration time per well, depending on firmware version. Fluorescence Polarization (FP) Glow Luminescence (Lumi) The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast,. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. These tools can be used in conjunction with SoftMax® Pro Software validation package and IQ/OQ/PQ validation protocols for FDA 21 CFR Part 11 compliance. A single software solution addresses your needs, from plate set-up to image analysis. Spectranet customer portal. The FlexStation 3 reader exclusively offers fluid transfer validation using Molecular Devices patented PathCheck® Feature to quantify the integrated 8- and 16-channel pipettor head performance. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. 00. It was then read from the bottom on a SpectraMax ® i3 Multi-Mode Microplate Reader using a preconfigured protocol in SoftMax Pro Software with the settings indicated in Table 1. 0 × 107) RLU 0. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and. The instruments feature a bottom-read mode ideal for whole-cell-based assays, and may be SoftMax Pro GxP Software. 9. Title: F3/F5 Multi Mode Microplate Readers | Molecular Devices Author: Molecular Devices, LLC Subject: Learn how the F3/F5 Multi-mode microplate readers can utilize its filter sets to accurately perform measurements with up to five modes of detection. 1. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. United States Phone: 800-635-5577 408-747-1700. The most published microplate reader control and data analysis software. 1-2 sec. Microplate Readers. 747. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. 0 . We would like to show you a description here but the site won’t allow us. The plate reader can measure multiple readouts, including UV and. Molecular Devices, LLC. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Category 2 and 3 microplate readers use gradient heating as well. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. Automated microplate readers reduce manual manipulation and ensure consistent operation. M2. If the computer is connected to the Internet: Select Search Automatically for updated driver software. The content of this site is published by the site. Products : General, Microplate Readers. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. These robust readers have been placed in labs from. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. SpectraDrop High-Throughput Screening (HTS) Kit. Methods. Every step is optimized to simplify analysis and reporting to support our microplate readers. com Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Two different agonists, acetylcholine and carbachol, were tested. Molecular Devices SpectraMax Gemini XS microplate reader with laptop and software (Pre-owned) $5,500. Integrated stacker option. M5. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. 3860 N First Street San Jose, CA 95134. 5. plate type, read area and number of images per well, plus specify positive/negative wells. Restart the system. Find regional support. Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments. Reads standard cuvettes & 96-384 well plates from 190 to 1000 nm. Never order or replace filters to optimize an assay again!SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Microplate readers and controlling software enable walk away data analysis. With various on-board features and. SoftMax Pro 7. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. Import plate-based data from any vendor SoftMax Pro enables you to import data from acquisition sources other than a Molecular Devices microplate reader. BMG Labtech - THERMOstar. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pmFor the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Uncontrolled Copy for Training and Reference Purposes Only . Shop now. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. Molecular Devices Flourmeteric Imaging. 635. After centrifugation, the plate was immediately read on the M5e and i3x readers using the instrument settings listed in Table 1. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Fluorescence Microplate Reader Comparison Chart. The absorbance at 412 nm was measured by a SpectraMax i3x Multi-Mode Microplate Reader ( Molecular Devices, San Jose, CA, USA) at 30 °C. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Image courtesy of Molecular Devices. Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. The drawing shows a standard microplate of 15 mm height covered with a lid. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. 0%, raw data units = counts/sec. Services. Supplier: Molecular Devices. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 5 sec. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. Gemini EM is a microplate spectrofluorometer from Molecular Devices. The Back Panel Figure 1. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Learn how you can utilize microplate readers to drive landmark discoveries. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. S. Contact our support team with your questions regarding Molecular Devices products and applications. 58×38×22 cm (22. Microplates for Molecular Devices Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. The sample is typically placed in a cuvette through which light is sent horizontally. D 1. Detection cartridge modularity allows for easy addition of new read modes within minutes without. . Integrate . Prior to fluorescence analysis, the lid from the OmniTray plate was removed. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. 4: Test tube cover. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. Search. For cryo preservation cells were scaled up in 10-layer factories (Corning Cat. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. 40-plate magazine (pair) 0200-6017. View the Molecular Devices website. Pre-defined protocols. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. S. For nearly 30 years, Molecular Devices has provided scientists with . 9. Some familiarity with Windows should be sufficient to use it. Tests every subsystem of the reader or . Endpoint. It also integrates with the. Molecular DevicesThe Marker Expression Protocol is used to identify cell areas labeled with the fluorescein-conjugated anti-VCAM antibody. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Note: removal of medium, followed by addition of a 1X solution of calcein AM and EthD-III, is. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. Explore our fully-integrated,. Mode Reader with the StakMax Microplate Handling System from Molecular Devices. The instruments feature a bottom-read mode ideal for whole-cell-based assays, and may beSoftMax Pro GxP Software.